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Infiltration of tumor cells into the central nervous system (CNS) is a major problem in brain tumor therapy. However, the mechanism of infiltration remains unclear. In this study, we investigated whether tumor necrosis factor (TNF) can induce intercellular adhesion molecule-1 (ICAM-1), which mediates adhesive interactions in the CNS. TNF added to human T cell lymphoma cell line (Karpas 299) or glioma cell line (U251MG) cells caused a dose-dependent increase in ICAM-1 mRNA levels. TNF-induced ICAM-1 mRNA increased transiently, peaked at 2 h and decreased by 6 h after TNF treatment. TNF-induced ICAM-1 gene expression was partially inhibited by a combination of actinomycin D, cycloheximide, and pentoxifylline. Human TNF receptor type 1 (TNFR1) protein was constitutively expressed in U251MG, Karpas 299, and NH7 cell lines. Human TNF receptor type 2 (TNFR2) protein was constitutively expressed only in U251MG cells. TNF induced TNFR2, but not TN


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