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Category: Graphic designThe typical skin of an adult human contains a population of stem cells that reside in the hair follicle (HF). These HF stem cells, or bulge cells, are progenitor cells that have the potential to both expand and differentiate into the hair follicle lineage. Previous studies have demonstrated that putative HF stem cells can be isolated from adult human scalp tissue. However, little is known about the properties of these cells in vivo.
Current approaches for the in vitro culture of HF stem cells involve methods that do not accurately reflect in vivo conditions. For instance, one in vitro approach involves growing cells in a scaffold material, while another approach involves growing cells on a tissue-like support material. See, e.g., Patel et al., 2015, Growth of Human Hair Follicle Stem Cells on a Dermatan Sulfate Derived Artificial Neural Network, Neuron, 94(2):328-340. Further, even when culture methods are used that attempt to more accurately mimic in vivo conditions, e.g., the use of substrates with cell surface receptor ligands or substrates with extracellular matrices, the cells become non-specific and do not maintain hair follicle lineage traits in vitro.
Thus, there remains a need in the art to develop methods for growing HF stem cells and/or hair follicle germs and/or cells, wherein said methods more accurately approximate in vivo conditions.Q:

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