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January 29, 2019
Editor: ArticleWorldTeamBiogenesis of yeast vacuoles. V. Inhibition of vacuolar hydrolysis of leucine and α-amino isobutyric acid.
The pH of vacuoles purified from Saccharomyces cerevisiae can reach pH 5.5 at a pH 4.5 external medium. The addition of vacuoles prepared from either vacuoles or vacuoles pretreated at low pH to a pH 4.5 medium did not reveal a significant acidification. In contrast, pretreatment of the vacuoles at pH 7.0 or higher resulted in a drastic decrease in the internal pH of the vacuoles. The vacuoles were then incubated with [14C]leucine or [14C]α-amino isobutyric acid. Only vacuoles pretreated at pH 7.0 and higher hydrolyzed more leucine than the controls, indicating that at pH greater than 6.5, vacuolar protons were sequestered and, consequently, pH-dependent vacuolar hydrolases were inactivated. The hydrolysis of α-am
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Recent advances in surgical techniques make it possible to repair simple and complex congenital heart disease. It is necessary to consider both the cosmetic result and the recovery of cardiac function. We have carried out a two-stage repair of congenital heart disease. In patients with pulmonary stenosis, the left ventricular outflow tract (LVOT) is closed with a valved patch and the conduit is reconstructed using a native pulmonary artery to create a new atrial communication. In patients with tetralogy of Fallot, the pulmonary artery is reconstructed and the ventricular septal defect is closed directly. In patients with intracardiac shunts, a previously placed conduit is renewed with a new conduit using a subclavian flap or a
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